These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Differential solubilization of proteins, phospholipids, free and esterified cholesterol of rat liver cellular membranes by sodium deoxycholate.
    Author: Ehrhart JC, Chauveau J.
    Journal: Biochim Biophys Acta; 1975 Feb 14; 375(3):434-45. PubMed ID: 1122282.
    Abstract:
    1. Smooth microsomes, Golgi-rich fractions, and light and heavy plasmalemmal subfractions from rat liver were isolated and their purity assessed using enzymic, chemical and morphological criteria. 2. Membranes were prepared by Tris-EDTA washing combined with sonication treatment of the different subcellular fractions. 3. Washed membranes were submitted to differential solubilization with 0.26% sodium deoxycholate. When the deoxycholate/phospholipid molar ratio (R) is raised, all the membranes showed a maximum protein solubilization occurring at R approximately equal TO 2. The higher the membrane neutral lipid to phospholipid molar ratio is, the lower the solubilized protein plateau lies. 4. Phospholipids are solubilized in slightly greater amounts than proteins and their solubilization is complete at R equals 14-16. 5. For R smaller than 2, sterols are solubilized in slightly greater amounts than phospholipids. At maximum protein solubilization, cholesterol and cholesterol esters completely differ in their behaviour. The whole membrane cholesterol goes into solution for R equals 14-16 while the solubilization of esterified cholesterol is never complete. The higher the protein plateau is, the lower the cholesterol esters solubilization curve asymptote lies.
    [Abstract] [Full Text] [Related] [New Search]