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  • Title: Intermolecular interactions between the SH3 domain and the proline-rich TH region of Bruton's tyrosine kinase.
    Author: Hansson H, Okoh MP, Smith CI, Vihinen M, Härd T.
    Journal: FEBS Lett; 2001 Jan 26; 489(1):67-70. PubMed ID: 11231015.
    Abstract:
    The SH3 domain of Bruton's tyrosine kinase (Btk) is preceded by the Tec homology (TH) region containing proline-rich sequences. We have studied a protein fragment containing both the Btk SH3 domain and the proline-rich sequences of the TH region (PRR-SH3). Intermolecular NMR cross-relaxation measurements, gel permeation chromatography profiles, titrations with proline-rich peptides, and (15)N NMR relaxation measurements are all consistent with a monomer-dimer equilibrium with a dissociation constant on the order of 60 microM. The intermolecular interactions do, at least in part, involve proline-rich sequences in the TH region. This behavior of Btk PRR-SH3 may have implications for the functional action of Btk.
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