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  • Title: alpha-Amylase production in high cell density submerged cultivation of Aspergillus oryzae and A. nidulans.
    Author: Agger T, Spohr AB, Nielsen J.
    Journal: Appl Microbiol Biotechnol; 2001 Jan; 55(1):81-4. PubMed ID: 11234963.
    Abstract:
    The effect of biomass concentration on the formation of Aspergillus oryzae alpha-amylase during submerged cultivation with A. oryzae and recombinant A. nidulans strains has been investigated. It was found that the specific rate of alpha-amylase formation in chemostats decreased significantly with increasing biomass concentration in the range of approx. 2-12 g dry weight kg(-1). When using a recombinant A. nidulans strain in which the gene responsible for carbon catabolite repression of the A. oryzae alpha-amylase gene (creA) was deleted, no significant decrease in the specific rate of alpha-amylase formation was observed. On the basis of the experimental results, it is suggested that the low value of the specific alpha-amylase productivity observed at high biomass concentration is caused by slow mixing of the concentrated feed solution in the viscous fermentation medium.
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