These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Effect of urotensin II on the airway smooth muscle cell proliferation and its mechanism].
    Author: Chen Y, Zhao M, Xia C.
    Journal: Zhonghua Yi Xue Za Zhi; 2000 Dec; 80(12):928-30. PubMed ID: 11236632.
    Abstract:
    OBJECTIVE: To investigate the effect and mechanism of Urotensin II on the airway smooth muscle cell proliferation. METHODS: (1) Using 3H-TdR incorporation to determine the effect of Urotensin II on the rat airway smooth muscle cells DNA synthesis. Different inhibitors were used to study the role of different signal transduction pathway such as protein kinase C (PKC), mitogen-activated protein kinase (MAPK), Calcineurin (CaN), Calmodulin-dependent protein kinase (CaM-PK) and calcium channel in the mitogenic effect of Urotensin II on the airway smooth muscle cells. (2) Using Fura-2/AM to measure the effect of Urotensin II on the cytosolic free calcium concentration. RESULTS: (1) Urotensin II (10(-10)-10(-6) mol/L) increased the airway smooth muscle cell 3H-TdR incorporation in a dose-dependent manner and Urotensin II 10(-6) mol/L reached the maximal effect. It was seven times as high as that of control (P < 0.01). (2) H7, PD98059, and nicardipine, inhibitors of PKC, MAPK and calcium channel, significantly inhibited Urotensin II (10(-7) mol/L)-stimulated airway smooth muscle cell 3H-TdR incorporation, with the inhibitory rate of 29% (P < 0.05), 45% (P < 0.01), and 28% respectively (P < 0.05). W7, an inhibitor of CaM-PK, had no effect (P > 0.05). (3) Cyclosporin A (10(-8)-10(-6) mol/L), inhibitor of CaN, an inhibited the airway smooth muscle cell 3H-TdR incorporation induced by Urotensin II (10(-7) mol/L) in a dose-dependent manner, with the inhibitory rate of 76% at 10(-6) mol/L (P < 0.01). (4) Urotensin II (10(-6) mol/L) promoted cytosolic free calcium concentration increase by 18% (P < 0.01). CONCLUSION: The effect of Urotensin II-stimulated airway smooth muscle cells DNA synthesis is mediated by Ca2+, PKC, MAPK and CaN signal transduction pathway.
    [Abstract] [Full Text] [Related] [New Search]