These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Altered membrane-cytoskeleton linkage and membrane blebbing in energy-depleted renal proximal tubular cells.
    Author: Chen J, Wagner MC.
    Journal: Am J Physiol Renal Physiol; 2001 Apr; 280(4):F619-27. PubMed ID: 11249853.
    Abstract:
    The effects of energy depletion on two membrane-cytoskeletal linker proteins (ezrin and myosin-1 beta) and membrane bleb formation were studied in isolated rabbit proximal tubule cells. Measurements of cytoskeletal-membrane interactions by using the laser optic trap method revealed a stronger association of control tubule membrane with the apical cytoskeleton compared with the basal cytoskeleton. Energy depletion weakened the apical membrane-cytoskeleton interactions to a greater degree. Biochemical studies demonstrated that energy depletion altered both ezrin and myosin-1 beta. The salt-insensitive ezrin fraction dissociated from the cytoskeleton; myosin-1beta redistributed from the peripheral cytoskeleton to a perinuclear/nuclear complex. These changes in ezrin and myosin-1 beta and the weakening of the membrane-cytoskeleton interactions correlated with the release of brush-border membrane blebs observed by differential interference contrast microscopy. Permeability of membrane blebs was also evaluated during energy depletion and indicated an increased permeabilization of basal blebs to 3-kDa dextrans. These results support the hypothesis that alterations in membrane-cytoskeleton linkers facilitate the formation and detachment of blebs by weakening membrane-cytoskeleton interactions.
    [Abstract] [Full Text] [Related] [New Search]