These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes.
    Author: Liu X, Zheng SX, Zhou LJ, Zhu XZ.
    Journal: Acta Pharmacol Sin; 2000 Jan; 21(1):19-22. PubMed ID: 11263242.
    Abstract:
    AIM: To examine the effect of recombinant human basic fibroblast growth factor (bFGF) on the expression of vascular endothelial growth factor (VEGF) in primary cultured rat astrocytes. METHODS: Semiquantification PCR (SQ-PCR) and immunocytochemistry were used to investigate the effect of bFGF on VEGF mRNA level and protein level, respectively. RESULTS: Treatment with bFGF dose-dependently increased the VEGF mRNA level in astrocytes. The up-regulation of VEGF mRNA induced by bFGF (10 micrograms/L) was detected as short as 3-h treatment. The increase of VEGF mRNA level reached the maximum after 24-h treatment with bFGF. The immunocytochemical staining showed that the VEGF protein level in astrocytes also increased after the cells were incubated with bFGF. CONCLUSION: bFGF induced a marked time- and concentration-dependent increase in VEGF expression in primary cultured astrocytes, suggesting that the effect of bFGF on angiogenesis in brain may act partly by up-regulating VEGF expression in astrocytes.
    [Abstract] [Full Text] [Related] [New Search]