These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Polymerase chain reaction associated with dig-labelled DNA hybridization of mip gene to identify new isolated Legionella pneumophila strains].
    Author: Lu X, Peng X, Ren H.
    Journal: Zhonghua Jie He He Hu Xi Za Zhi; 1998 Mar; 21(3):154-6. PubMed ID: 11263351.
    Abstract:
    OBJECTIVE: By testing mip gene, one highly conservative virulence gene of Legionella pneumophila (Lp), as targets of polymerase chain reaction (PCR) and Digoxin-labelled probe hybridization to establish a rapid, specific and sensitive gene analysis method that can identify new isolated suspected bacteria strains as Lp. METHOD: Abstracting all the CDC reference Lp strains' and none-Lp Legionella strains' DNA, as well as the domestic isolated Lp strains' and some non-Legionella control bacteria's DNA as templates, then were processed. All the positive PCR products(if no PCR amplicons were available, the original whole DNA should be detected) were hybridized with Digoxingen-labelled mip gene probe in dot-blot procedure. RESULT: All the tested Lp strains had positive PCR and hybridization results, at the same time, all none-Legionella bacteria and none-Lp Legionella strains got negative results. 6 of 26 isolates were identified as Lp strains by this method. CONCLUSION: Such a Lp strain identification procedure shows high specificity and sensitivity (nearly 100%, in this study), and mean-while can be completed in a relative short time. Surely, this method can be largely available and has a potential value in diagnosis of clinical Legionellosis cases and pursuing the pathogen during Legionnaires' disease outbreaks.
    [Abstract] [Full Text] [Related] [New Search]