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  • Title: [Detection of mycobacterium tuberculosis by AmpliSensor-PCR technique and its clinical application].
    Author: Tan Y, Li Y, Tan S.
    Journal: Zhonghua Jie He He Hu Xi Za Zhi; 1998 Feb; 21(2):79-81. PubMed ID: 11263388.
    Abstract:
    OBJECTIVE: To explore the value of Amplisensor-PCR method in diagnosis of tuberculosis. METHOD: The samples from 784 patients with tuberculosis, 160 patients with lung cancer were detected by using AmpliSensor-PCR, PCR, smear and culture methods, and the results were compared. RESULT: The sensitivity of AmpliSensor-PCR technique was obviously higher than that of smear and culture (P < 0.01). The specificity of AmpliSensor-PCR method was higher than that of PCR method. CONCLUSION: Amplisensor-PCR method can be determined to detect the lower limit by standard curve, through which the original amount of target-DNA in samples can be calculated. AmpliSensor-PCR method shows higher specificity and sensitivity. It is of clinical value in diagnosis of pulmonary tuberculosis and particularly of extrapulmonary tuberculosis.
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