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  • Title: Inhibition of Tat transactivation by the RNA polymerase II CTD-phosphatase FCP1.
    Author: Licciardo P, Napolitano G, Majello B, Lania L.
    Journal: AIDS; 2001 Feb 16; 15(3):301-7. PubMed ID: 11273209.
    Abstract:
    OBJECTIVES: To asses the role of the RNAPII carboxy-terminal domain (CTD) phosphatase FCP1 on HIV-1 Tat-mediated transactivation. DESIGN: Construction of expression vectors encoding FCP1 phosphatase and analysis of their functions on Tat activity. METHODS: Basal and Tat-mediated transactivation of HIV-1 long terminal repeat (LTR)-driven transcription was compared, by transient transfections, in the presence of FCP1 phosphatase. Protein interactions were analysed by in vitro binding assays. RESULTS: FCP1 specifically and effectively represses Tat transactivation but not HIV-1 LTR-basal transcription. Protein interaction assays demonstrated that FCP1 specifically and directly binds Tat in vitro. CONCLUSION: The specific and efficient inhibitory function of FCP1 highlights the important role of this CTD-phosphatase in Tat-mediated transactivation, and it suggests that FCP1 might represent a specific target for modulation of Tat activity in infected cells.
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