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  • Title: [Intestinal flora of patients with suspected antibiotic associated diarrhea (AAD). I. Clostridium perfringens].
    Author: Pituch H, Martirosian G, Obuch-Woszczatyński P, Meisel-Mikołajczyk F, Łuczak M.
    Journal: Med Dosw Mikrobiol; 2000; 52(4):375-82. PubMed ID: 11286179.
    Abstract:
    Stool samples of 158 patients suspected of antibiotic-associated diarrhoea (AAD) were studied. Toxin A of C. difficile and enterotoxin of C. perfringens were detected in stool samples by immunoenzymatic assays and PCR. In 35 stool samples toxin A of C. difficile was detected and in 48 cases (30%) C. difficile strains were cultured from 21 stool samples (13%). The presence of the cpe gene of C. perfringens, enabling the production of enterotoxin, could not be detected by PCR, both in stool samples and in isolated strains, using ent 1 and ent 2 primer pairs. C. difficile and C. perfringens were isolated from the same stool samples in 4 cases. From stool samples of two patients with AAD C. perfringens strains, thermoresistant spores were cultured.
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