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  • Title: Rapid and sensitive biosensor for Salmonella.
    Author: Pathirana ST, Barbaree J, Chin BA, Hartell MG, Neely WC, Vodyanoy V.
    Journal: Biosens Bioelectron; 2000 Jun; 15(3-4):135-41. PubMed ID: 11286330.
    Abstract:
    The rapid and sensitive detection of Salmonella typhymurium based on the use of a polyclonal antibody immobilized by the Langmuir-Blodgett method on the surface of a quartz crystal acoustic wave device was demonstrated. The binding of bacteria to the surface changed the crystal resonance parameters; these were quantified by the output voltage of the sensor instrumentation. The sensor had a lower detection limit of a few hundred cells/ml, and a response time of < 100 s over the range of 10(2)-10(10) cells/ml. The sensor response was linear between bacterial concentrations of 10(2)-10(7) cells/ml, with a sensitivity of 18 mV/decade. The binding of bacteria was specific with two binding sites needed to bind a single cell. The sensors preserve approximately 75% of their sensitivity over a period of 32 days.
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