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  • Title: Identification of proteins from Escherichia coli using two-dimensional semi-preparative electrophoresis and mass spectrometry.
    Author: Gustafsson E, Thorén K, Larsson T, Davidsson P, Karlsson KA, Nilsson CL.
    Journal: Rapid Commun Mass Spectrom; 2001; 15(6):428-32. PubMed ID: 11291121.
    Abstract:
    Escherichia coli is a gram-negative bacterium that causes sepsis and infections of the nervous system, and the digestive and urinary tracts. The availability of the complete nucleotide sequence encoding the E. coli K-12 genome has made this organism an excellent model for proteomic studies. Semi-preparative two-dimensional electrophoresis, including liquid phase isoelectric focusing (IEF), one-dimensional sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and gel elution, have for the first time been used in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS), electrospray tandem mass spectrometry and database searching for rapid separation of proteins from a uropathogenic strain of E. coli. The identity of 30 proteins, including the membrane protein nmpC, was obtained using this approach.
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