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  • Title: Improved hatch rate in helium-oxygen by reducing shell diffusion area.
    Author: Weiss HS.
    Journal: Proc Soc Exp Biol Med; 1975 Mar; 148(3):937-41. PubMed ID: 1129317.
    Abstract:
    For eggs incubating in a He atmosphere (79% He/21% O2), covering approximately 50% of the shell with melted paraffin improves hatch rate to control values (from 20% to 74%) and decreases egg weight loss to control values (from 17% to 9%). In air (79% N2/21% O2) the same paraffin treatment depresses hatch rate. The role of the inert gases in incubation appears to be an indirect one related to their modification of the rate of gaseous flux across the shell with the adverse effects of He due to excessively rapid diffusion.
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