These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Immunolocalization of different tubulin epitopes in the spermatozoon of Bacillus rossius (Insecta, Phasmatodea).
    Author: Taddei AR, Gambellini G, Fausto AM, Baldacci A, Mazzini M.
    Journal: J Submicrosc Cytol Pathol; 2000 Oct; 32(4):635-43. PubMed ID: 11297383.
    Abstract:
    The existence of distinct tubulins in microtubules forming the sperm axoneme has been demonstrated in various species, whereas little is known about the distribution of tubulin variants in insect spermatozoa. In the present study, a panel of specific antibodies has been used to investigate the presence and localization of tubulin isotypes and post-translationally modified tubulins in the spermatozoon of the stick insect Bacillus rossius. Indirect immunofluorescence and immunogold staining showed differences in labelling in the mature sperm and that the tubulin epitopes localized differentially in the axoneme. In particular, the tyrosinated alpha-tubulin mainly occurs on doublets. These results provide an insight into the molecular composition of the microtubules forming the sperm axoneme of B. rossius and suggest that the structural specificity could reflect distinct functional roles within axonemal microtubules.
    [Abstract] [Full Text] [Related] [New Search]