These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Determination of two components in chloramphenicol and hydrocortisone ear drops by high performance liquid chromatography].
    Author: Li X.
    Journal: Se Pu; 1998 Jan; 16(1):71-3. PubMed ID: 11324485.
    Abstract:
    Chloramphenicol and hydrocortisone ear drops are possessed of anti-inflammatory and antiallergic functions. It can be used effectively in the treatment of tympanitis. It is composed of chloramphenicol and hydrocortisone. In this paper, we report a reversed phase HPLC method for the determination of chloramphenicol and hydrocortisone in the drops. The operatings conditions were YWG-C18 column (5 microns, 5 mm x 150 mm) at 30 degrees C, mobile phase of methanol-water (60:40, V/V) and UV detector at 244 nm. Prednisone acetate was selected as the internal standard. The linear relationship of calibration curves was good in the range of 40-200 and 8-40 mg/L for chloramphenicol and hydrocortisone, respectively (r = 0.9999). The average recovery and RSD of chloramphenicol and hydrocortisone were 99.91%, 0.86% and 99.58%, 1.34% (n = 6) respectively. The total time for separation and determination was within 9 min. The method was simple, sensitive, rapid and accurate.
    [Abstract] [Full Text] [Related] [New Search]