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  • Title: Light and electron microscopic investigation of the lectin-binding pattern in the oxyntic gland region of bovine abomasum.
    Author: Sommer U, Rehn B, Kressin M.
    Journal: Ann Anat; 2001 Mar; 183(2):135-43. PubMed ID: 11325060.
    Abstract:
    For the first time the expression of glycoconjugate residues in the oxyntic gland region of bovine abomasum has been investigated by means of lectin histochemistry. For light microscopic investigations, a battery of ten lectins, Con A, PSA, UEA I, WGA, LEA, SNA, RCA120, MPA, DBA and SBA was used. For electron microscopic examinations, WGA and RCA120 were utilized. The staining pattern of the lectins in all exocrine cell types of the oxyntic gland region is described. Compared to the results of monogastric species our study reveals some similarities, but just as many differences in the composition of glycoconjugate residues in bovine exocrine cell types. Typical for surface mucous cells is the amount of L-fucose, N-acetyl glucosamine residues and Galbeta1, 4GlcNAc sequences in the secretory granules. SNA could serve as a marker for surface mucous cells, because this lectin exclusively stains the plasma membrane and the secretory granules of surface mucous cells and the extracellular mucus. L-fucose and N-acetyl glucosamine are typical for the secretory granules of mucous neck cells. In addition, the secretory granules show the highest amount of N-acetyl galactosamine residues of all exocrine cells, so that DBA and SBA are recommended as marker lectins for mucous neck cells. Most lectins strongly stain the intracellular membrane system of oxyntic cells. The cocktail of glycoconjugates in the vicinity of the HCI production site provide protection against chemical injury. In chief cells only the apical plasma membrane is more or less labeled with all lectins apart from SNA. Specific marker lectins for oxyntic cells or chief cells of the bovine have not been characterized.
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