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  • Title: Thermally induced changes of lipoate acetyltransferase inner core isolated from the Bacillus stearothermophilus pyruvate dehydrogenase complex.
    Author: Aso Y, Nakajima A, Meno K, Ishiguro M.
    Journal: Biosci Biotechnol Biochem; 2001 Mar; 65(3):698-701. PubMed ID: 11330694.
    Abstract:
    Incubation at 70 degrees C converted the Bacillus stearothermophilus lipoate acetyltransferase inner core into an unidentified active molecular form, X, yielding an inactive aggregate. The core and X showed similar thermostabilities, but they were different in the recovery of enzyme activity after incubation with 1.2-2.0 M guanidine hydrochloride and its subsequent removal; the core was hardly recovered, but X was well recovered.
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