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  • Title: cDNA sequence and gene expression of storage protein-2--a juvenile hormone-suppressible hexamerin from the fall webworm, Hyphantria cunea Drury.
    Author: Hwang SJ, Cheon HM, Kim HJ, Chae KS, Chung DH, Kim MO, Park JS, Seo SJ.
    Journal: Comp Biochem Physiol B Biochem Mol Biol; 2001 May; 129(1):97-107. PubMed ID: 11337253.
    Abstract:
    We isolated and sequenced a cDNA clone corresponding to storage protein-2 (SP-2) from the fall webworm, Hyphantria cunea. The cDNA for SP-2 (2572 bp) codes for a 747-residue protein with a predicted molecular mass of 88.5 kDa. The calculated isoelectric point is 7.6. Multiple alignment analysis of amino acid sequence revealed that SP-2 is most similar to BJHSP2 (74.3% identity). According to both the phylogenetic analyses and criteria for amino acid composition, SP-2 belongs to the subfamily of moderately methionine-rich storage proteins (3.2% methionine, 11.8% aromatic amino acid). Topical application of the JH analog, methoprene, after head ligation of larvae, suppressed transcription of the SP-2 gene, indicating hormonal effects at the transcriptional level. The SP-2 transcript was detected by Northern blot analysis in Malpighian tubules, in addition to the fat body where it was most abundant. The local expression of SP-2 in Malpighian tubules suggests that it may have some function in that organ.
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