These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Release of catecholamines and dopamine beta-hydroxylase from the perfused adrenal gland of the cat.
    Author: Dixon WR, Garcia AG, Kirpekar SM.
    Journal: J Physiol; 1975 Jan; 244(3):805-24. PubMed ID: 1133780.
    Abstract:
    1. Secretion of catecholamines (CA) and dopamine beta-hydroxylase (DBH) activity from the perfused cat adrenal gland was studied following splanchnic nerve stimulation or infusion of acetylcholine (ACh). 2. Splanchnic nerve stimulation (30 Hz) or perfusion with a low concentration of ACh (10-minus5 M) caused a marked release of CA in the venous effluent, but release of DBH activity was minimal while a higher concentration of ACh (10-minus 4 M) enhanced the release of CA and DBH. 3. The ratio of DBH/CA released in the perfusate by splanchnic nerve stimulation or ACh infusion was only a small fraction of the ratio in the soluble lysate of purified chromaffin vesicles. 4. Following reserpine treatment, adrenal CA levels fell to 25% of the control value in 24 hr, remained depressed on days 2, 3, 4 and 5 at 5% of the control and recovered to 60% of the control value on the 6th day. DBH activity was unchanged from the control value at 24 hr after treatment, then rose as high as 5 times the control on the 5th day and was still twice the control value on the 6th day. 5. CA secretion in response to ACh (10-minus 4 M) perfusion was reduced to 30% of the control value on the first day after reserpine treatment, while DBH secretion was unchanged. On the 2nd day, CA secretion was depressed further to 5% of the control and remained at this low level up to 5 days after treatment while DBH secretion was twice the control value at 48 hr and then on days 3, 4 and 5 rose up to 5 times the control value. On the 6th day, secretion of CA recovered to 30% of the control while DBH secretion was now twice the control. 6. Isopycnic sucrose density (discontinuous) gradient centrifugation of vesicles from adrenal glands of control cats, and of cats given reserpine 1 or 2 days perviously, indicated that new vesicles or vesicles depleted of CA by reserpine had a lower equilibrium density than the original population of vesicles. 7. These results suggest that the release of CA is quantal in nature, but the release of DBH is not necessarily coupled with it. Release of DBH by ACh from reserpinized glands suggests that the vesicles which were once involved in secretion may be re-used for synthesis and storage of CA.
    [Abstract] [Full Text] [Related] [New Search]