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Title: Nucleotide and deduced amino acid sequences of an alkaline pullulanase from the alkaliphilic bacterium Bacillus sp. KSM-1876. Author: Hatada Y, Saito K, Hagihara H, Ozaki K, Ito S. Journal: Biochim Biophys Acta; 2001 Feb 09; 1545(1-2):367-71. PubMed ID: 11342061. Abstract: The nucleotide sequence of an alkaline pullulanase-encoding gene from alkaliphilic Bacillus sp. strain KSM-1876 was determined. The open reading frame of the gene encoded 1142 amino acids with a calculated molecular mass of 128739 Da. The alkaline pullulanase showed very limited homology (<32% identity) to previously reported debranching enzymes from prokaryotes and eukaryotes. It contained unique tandem repeats in both the N-terminal and the C-terminal regions.[Abstract] [Full Text] [Related] [New Search]