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  • Title: The uptake of glucose and gluconate by Pseudomonas putida.
    Author: Vicente M, Pedro MA, Torrontegui G, Cánovas JL.
    Journal: Mol Cell Biochem; 1975 Apr 30; 7(1):59-64. PubMed ID: 1134500.
    Abstract:
    The uptake of glucose and gluconate is under inductive control in Pseudomonas putida. Glucose, gluconate, and 2-ketogluconate were each good nutritional inducers of these transport abilities. Glucose and gluconate uptake obeyed saturation kinetics: the apparent Km for glucose was 6 mM and that for gluconate was 0.5 mM. Therefore, transport of both substrates appears to be mediated by enzyme-like carriers. Glucose and gluconate are parallel inhibitors for their uptake9 Strains selected for their inability totransport glucose were found to be deficient in gluconate uptake. The reverse was alsotrue: mutations affecting gluconate entry also blocked the uptake of glucose. These results demonstrate that a common carrier is involved in the uptake of both glucose and gluconate by P. putida cells.
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