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  • Title: A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
    Author: Kearney JF, Radbruch A, Liesegang B, Rajewsky K.
    Journal: J Immunol; 1979 Oct; 123(4):1548-50. PubMed ID: 113458.
    Abstract:
    We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.
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