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  • Title: Effect of cyclic stretch on alpha-subunit mRNA expression of Na+-K+-ATPase in aortic smooth muscle cells.
    Author: Sevieux N, Alam J, Songu-Mize E.
    Journal: Am J Physiol Cell Physiol; 2001 Jun; 280(6):C1555-60. PubMed ID: 11350750.
    Abstract:
    We previously demonstrated that protein expression of both alpha(1)- and alpha(2)-catalytic subunits of the Na+-K+-ATPase is elevated after a 2- to 4-day chronic cyclic stretch regimen in cultured aortic smooth muscle cells (ASMC). In the present study, we investigated whether cyclic stretch affects mRNA expression of the alpha-isoforms of the Na+-K+-ATPase. Using a stretch apparatus, rat ASMC were cyclically stretched 10 or 20% of their length for 1, 3, or 6 h. alpha-Isoform mRNA levels were measured using Northern analysis. A 3-h 10% stretch had no significant affect on mRNA expression for either isoform, but a 20% stretch increased mRNA of both isoforms approximately twofold. Whereas a 6-h 20% stretch increased alpha(1) mRNA by 3.3-fold, alpha(2) was not affected any further. Actinomycin D blocked the stretch-induced stimulation of mRNA expression of both alpha-subunits. In conclusion, cyclic stretch stimulates the mRNA expression of both alpha(1)- and alpha(2)-subunits of Na+-K+-ATPase. The sensitivity of the two genes to the degree and duration of stretch is different. The stretch-induced increase of mRNA may be a result of increased transcription.
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