These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: The synthesis of prostaglandin F by human endometrium in organ culture.
    Author: Cane EM, Villee CA.
    Journal: Prostaglandins; 1975 Feb; 9(2):281-8. PubMed ID: 1135440.
    Abstract:
    Slices of human endometrium obtained from hysterectomy specimens were cultured for 48 hours in an organ culture medium supplemented with ethanol (control, vehicle), 17-beta-estradiol (.5 mug/ml), or progesterone (.5 mug/ml). Uncultured endometrial tissue, cultured tissues, and the media were assayed for prostaglandin F (PGF) by a radioimmunoassay technique. Hematoxylin and eosin and periodic acid-Schiff stain histologic controls were done on all tissues. The concentrations of PGF in picograms/milligram, corrected for percent recovery, in the differently treated tissues were: preculture 298; culture control 2210; estrogen-treated 2680; progesterone-treated 1260. All differences except those between estrogen and control (p greater than .10) and progesterone and control (p less than .10) are significant at the p = .02 level or better. Progesterone appears to inhibit PGF synthesis which occurs during in vitro culture of human endometrium; estrogen tended to increase PGF synthesis in this system. Slices of human endometrium obtained from hysterectomy specimens were cultured for 48 hours in an organ culture medium supplemented with ethanol (control, vehicle) 17-Beta-estradiol (.5 mc/ml), or progesterone (.5 mc/ml). Uncultured endometrial tissue, cultured tissues, and the media were assayed for prostaglandin F (PGF) by a radioimmunoassay technique. Hematoxylin and eosin and periodic acid-Schiff stain histologic controls were done on all tissues. The concentrations of PFG in picograms/milligram, corrected for % recovery, in the differently treated tissues were: preculture 298; culture control 2210; estrogen-treated 2680; progesterone-treated 1260. All differences except those between estrogen and control (P .10) and progesterone and control (p .10) are significant at the p=.02 level or better. Progesterone appears to inhibit PGF synthesis which occurs during in vitro culture of human endometrium estrogen tended to increase PGF synthesis in this system.
    [Abstract] [Full Text] [Related] [New Search]