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  • Title: Isozymes of alpha-amylase in human urine.
    Author: Minamiura N, Kimura Y, Tsujino K, Yamamoto T.
    Journal: J Biochem; 1975 Jan 01; 77(1?):163-9. PubMed ID: 1137983.
    Abstract:
    Some physicochemical properties of crystalline alpha-amylase [EC 3.2.1.1] isolated from normal human urine were investigated. A crystalline preparation of the enzyme was homogeneous on velocity sedimentation, gel filtration, and sodium dodecyl sulfate disc electrophoresis, and its molecular weight was estimated to be 4.5 X 10-4. However, electrophoretic analyses revealed that the crystalline alpha-amylase consisted of at least five isozymes. The implications of the experimental results are discussed.
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