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  • Title: Quantification of L-ascorbic acid and total ascorbic acid in fruits and spinach by capillary zone electrophoresis.
    Author: Liao T, Jiang CM, Wu MC, Hwang JY, Chang HM.
    Journal: Electrophoresis; 2001 May; 22(8):1484-8. PubMed ID: 11386660.
    Abstract:
    A standard curve for the quantification of L-ascorbic acid (L-AA) by capillary zone electrophoresis (CZE) was established, and the quantification of ascorbic acid and total ascorbic acid in fruits (lemon, Sunkist, and pineapple) and spinach were performed using D-isoascorbic acid (D-IAA) as an internal standard. The minimum detection limits (MDLs) for L-AA and D-IAA were determined to be 1 and 2 microg/mL, respectively, at 265 nm. Dehydroascorbic acid (DHAA) in fruits and spinach was quantified in the presence of DL-homocysteine. The recoveries for L-AA in these juices were between 95 and 105%.
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