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  • Title: Expression and purification of Escherichia coli beta-glucuronidase.
    Author: Aich S, Delbaere LT, Chen R.
    Journal: Protein Expr Purif; 2001 Jun; 22(1):75-81. PubMed ID: 11388802.
    Abstract:
    A strong and constitutive expression vector of Escherichia coli beta-glucuronidase with the isocitrate dehydrogenase promoter has been developed for producing a large amount of recombinant protein. More than 95% pure enzyme was obtained by a four step purification procedure-ammonium sulfate precipitation, DEAE ion-exchange chromatography, Superose 12 gel filtration, and hydroxyapatite steric ion-exchange chromatography. The overexpressed gene can produce 23 mg of pure enzyme from one liter of bacterial culture.
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