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  • Title: Inexpensive purification of P450 reductase and other proteins using 2',5'-adenosine diphosphate agarose affinity columns.
    Author: Rock D, Rock D, Jones JP.
    Journal: Protein Expr Purif; 2001 Jun; 22(1):82-3. PubMed ID: 11388803.
    Abstract:
    Two reductases, P450 oxidoreductase and P450Bm-3 reductase, were purified on a 2',5'-adenosine diphosphate solid support. Although the efficiency of these columns is well established, the cost of the resin and the eluting material 2'-adenosine can be prohibitive. Herein we show that the less costly 2',3'-adenosine monophosphate is an excellent eluting material.
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