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  • Title: [Primary structure of the cytoplasmic aspartate aminotransferases from the swine myocardium. Isolation, purification and characteristics of the peptides from cyanogen bromide cleavage].
    Author: Nosikov VV, Polianovskiĭ OL, Braunshtein AE, Grishin EV, Ovchinnikov IuA.
    Journal: Biokhimiia; 1975; 40(1):107-14. PubMed ID: 1138991.
    Abstract:
    Cytoplasmic aspartate aminotransferase from pig heart muscle was cleaved with cyanogen bromide and 8 peptide fragments were isolated. The high tendency of the large peptides for aggregation was overcome only by the utilization of special procedures of the denaturation and acylation of the lysine residues of peptide with citraconic anhydride. Peptides were separated by gel chromatography on sephadex G-50 and G-75 and by ion exchange chromatography on cellulose DE-22 and DE-32 with use of concentrated urea solutions. Amino acid composition and N-terminal residues of isolated peptides were determined.
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