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  • Title: The 5' flanking region of the human bone morphogenetic protein-7 gene.
    Author: Dorai H, Shepard A, Ozkaynak E, Lin K, Jones S, Oppermann H, Sampath K.
    Journal: Biochem Biophys Res Commun; 2001 Apr 06; 282(3):823-31. PubMed ID: 11401538.
    Abstract:
    We describe here the cloning and characterization of the 5' flanking region of the human Bone Morphogenetic Protein-7 (BMP-7) gene from a 3.3 kb genomic DNA fragment. Functional analysis by transient transfection using the luciferase reporter gene indicated that this region had a low basal promoter activity in human Wilm's tumor derived renal (G401) and rat osteoblast (ROS 17/2.8) cell lines. Sequential deletion analysis of the promoter revealed sequences whose presence correlated with decreased expression of the reporter gene. Coexpression of transcription factors involved in epithelial/mesenchymal interactions during kidney and eye development dramatically stimulated the expression of the reporter gene from the putative BMP-7 promoter. Finally, a subset of agents that upregulated the expression of the reporter gene from the cloned promoter were also shown to increase the expression of the endogenous BMP-7 in G401 and ROS cell lines in vitro.
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