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Title: Streptomyces matensis laminaripentaose hydrolase is an 'inverting' beta-1,3-glucanase. Author: Nishimura T, Bignon C, Allouch J, Czjzek M, Darbon H, Watanabe T, Henrissat B. Journal: FEBS Lett; 2001 Jun 15; 499(1-2):187-90. PubMed ID: 11418137. Abstract: The laminaripentaose-producing beta-1,3-glucanase of Streptomyces matensis is a member of the glycoside hydrolase family GH-64. We have constructed and purified a recombinant hexahistidine-tagged form of the enzyme for characterisation. The enzyme, which exists as a monomer in solution, hydrolyses beta-1,3-glucan by a mechanism leading to overall inversion of the anomeric configuration. This is the first determination of the mechanism prevailing in glycoside hydrolase family GH-64 and this is the first characterisation of an 'inverting' beta-1,3-glucanase.[Abstract] [Full Text] [Related] [New Search]