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  • Title: Determination of hydrogen sulfide and acid-labile sulfur in animal tissues by gas chromatography and ion chromatography.
    Author: Ubuka T, Abe T, Kajikawa R, Morino K.
    Journal: J Chromatogr B Biomed Sci Appl; 2001 Jun 05; 757(1):31-7. PubMed ID: 11419746.
    Abstract:
    A sensitive and reliable method was developed for the determination of hydrogen sulfide and acid-labile sulfur (ALS) in animal tissues using gas chromatography with flame photometric detector (GC-FPD) and ion chromatography (IC). Hydrogen sulfide trapped in alkaline solution was determined by GC-FPD as hydrogen sulfide or by IC as sulfate after oxidation with hydrogen peroxide. Sodium sulfide used as a source of hydrogen sulfide was standardized by IC. Fresh rat liver and heart tissues contained 112.2+/-23.0 and 274.1+/-34.6 nmol/g of ALS respectively. Free hydrogen sulfide was not detected.
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