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  • Title: Avidity analysis of the human immune response to a chitin binding protein of Toxoplasma gondii.
    Author: Villavedra M, Battistoni JJ, Rossi S, Carol H, Nieto A.
    Journal: Int J Parasitol; 2001 Aug; 31(10):1087-92. PubMed ID: 11429172.
    Abstract:
    A 30-33 kDa electroeluted fraction of T. gondii tachyzoites improved discrimination between acute and chronic phase sera when used instead of the whole tachyzoite extract in an avidity-ELISA. In order to identify the components of these fractions, crude tachyzoite antigen was fractionated by anionic exchange chromatography. The 30-33 kDa antigen cluster eluted in the not-bound fraction could account for a large proportion of the antibody response against the 30-33 kDa electroeluted fraction. According to the N-terminal sequence data, this antigen fraction is composed mainly of SAG1 and another protein with high homology to chitin binding proteins from plants.
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