These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Method to produce 9(S)-hydroperoxides of linoleic and linolenic acids by maize lipoxygenase.
    Author: Gardner HW, Grove MJ.
    Journal: Lipids; 2001 May; 36(5):529-33. PubMed ID: 11432467.
    Abstract:
    Seed from maize (corn) Zea mays provides a ready source of 9-lipoxygenase that oxidizes linoleic acid and linolenic acid into 9(S)-hydroperoxy-10(E),12(Z)-octadecadienoic acid and 9(S)-hydroperoxy-10(E),12(Z),15(Z)-octadecatrienoic acid, respectively. Corn seed has a very active hydroperoxide-decomposing enzyme, allene oxide synthase (AOS), which must be removed prior to oxidizing the fatty acid. A simple pH 4.5 treatment followed by centrifugation removes most of the AOS activity. Subsequent purification by ammonium sulfate fractional precipitation results in negligible improvement in 9-hydroperoxide formation. This facile alternative method of preparing 9-hydroperoxides has advantages over other commonly used plant lipoxygenases.
    [Abstract] [Full Text] [Related] [New Search]