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  • Title: Construction of an Escherichia coli knockout strain for functional analysis of tRNA(Asp).
    Author: McClain WH, Gabriel K.
    Journal: J Mol Biol; 2001 Jul 13; 310(3):537-42. PubMed ID: 11439021.
    Abstract:
    The specific aminoacylation of tRNA is critical for translation of the genetic code. A molecular description of aminoacylation requires knowledge of the relevant three-dimensional structures, biochemical parameters and the structure-function relationship of the synthetase and its substrate tRNA. Extensive structural and biochemical data are available on the aspartic acid system of Escherichia coli, but there is a paucity of cellular functional data. We have developed a system to overcome this deficiency by engineering an E. coli knockout tRNA(Asp) strain, thereby allowing a penetrating analysis of tRNA(Asp) structure and function under conditions that prevail in the cell.
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