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Title: [Isolation and identification of tyrosine kinase from the cytosolic fraction of bovine retinal rod outer segments]. Author: Sholukh AM, Artamonov ID, Baranova LA, Lipkin VM, Volotovskiĭ ID. Journal: Mol Biol (Mosk); 2001; 35(3):510-4. PubMed ID: 11443935. Abstract: It was shown that the cytosol fraction of bovine retinal rod outer segments contains three forms of tyrosine kinase. One of them was purified 171-fold to attain a specific activity of 1.6 nmol/min per mg protein. The isolated protein had a molecular weight of about 54,000 in SDS electrophoresis. It was shown that this protein is a tyrosine-specific protein kinase, capable of autophosphorylation at the tyrosine residues and restoration of kinase activity upon denaturation-renaturation.[Abstract] [Full Text] [Related] [New Search]