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  • Title: Calcium-specific precipitation of dentin phosphoprotein: a new method of purification and the significance for the mechanism of calcification.
    Author: Kuboki Y, Fujisawa R, Aoyama K, Sasaki S.
    Journal: J Dent Res; 1979 Sep; 58(9):1926-32. PubMed ID: 114563.
    Abstract:
    Calcium, but not magnesium or strontium ions, specifically induce the precipitation of dentin phosphoprotein, which is reversibly solubilized by EDTA. This finding is very useful in pruifying dentin phosphoprotein of either a free or matrix-bound type. Bovine dentin phosphoproteins, thus isolated, contain a minimal amount of contaminant protein, unlike previous preparations.
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