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  • Title: Detection of Coxsackie B virus IgM antibody in myocarditis patients by Mac ELISA.
    Author: Zhu H, Zhang LB.
    Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2000 Dec; 14(4):370-2. PubMed ID: 11471030.
    Abstract:
    OBJECTIVE: To detect Coxsackie B virus type 1-6 IgM antibodies from 193 clinically diagnosed myocarditis cases. METHODS: The capture IgM ELISA (Mac ELISA) test was used. RESULTS: The total positive rate of Coxsackie virus B 1-6 IgM antibody in myocarditis patients was 69.6% (135 cases), among which Cox B3 type was as high as 40% (54 cases) of the total positive patients. The others were 29.6% (40 cases), 18.5% (25 cases), 7.4%(10 cases), 3.0% (4 cases), 1.5% (2 cases) of Cox B2, Cox B4, Cox B1, Cox B5 and Cox B6 respectively. Meanwhile only 20.4% (37 cases) Cox B IgM positive cases could be detected in 181 non-myocarditis patients of same ages. The results showed that the positive rate of Cox B virus IgM antibody in clinically diagnosed myocarditis was significantly higher than that in control group (P <0.01). CONCLUSIONS: The Cox B viruses may be the main agents causing viral myocarditis in Beijing cases. Mac ELISA is an easy, rapid, specific and sensitive method to detect Cox B virus IgM antibody for early and rapid diagnosis of myocarditis.
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