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  • Title: Polymerase chain reaction detection of Treponema denticola in endodontic infections within root canals.
    Author: Siqueira JF, Rôças IN, Favieri A, Oliveira JC, Santos KR.
    Journal: Int Endod J; 2001 Jun; 34(4):280-4. PubMed ID: 11482139.
    Abstract:
    AIM: A 16S rDNA-based polymerase chain reaction (PCR) method was used to detect the occurrence of Treponema denticola in root canals. METHODOLOGY: Samples were collected from 54 single-rooted teeth having carious lesions and necrotic pulps. DNA extracted from the samples was amplified using the PCR assay, which yielded a specific fragment of T. denticola 16S rDNA. RESULTS: T. denticola was detected in 10 of 29 asymptomatic cases (34.5%), eight of 15 root canals that were tender to percussion (53.3%), and five of 10 teeth with acute periradicular abscess (50%). In general, T. denticola was found in 23 of 54 cases (42.6%). CONCLUSIONS: Findings suggest that T. denticola can be involved in the pathogenesis of periradicular lesions of endodontic origin.
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