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  • Title: Studies on lysine, glutamine and glutamic acid tRNAs from Drosophila.
    Author: White BN.
    Journal: Biochim Biophys Acta; 1975 Jul 07; 395(3):322-8. PubMed ID: 1148239.
    Abstract:
    The minor bases present in the family of Drosophila tRNAs recognising codons of the type NAA or NAG have been studied. Under standard aminoacylating conditions, the acceptor activities of BrCN-treated tRNA-Lys-5 tRNA-Glu-4 and tRNA-G1n-4 were completely eliminated, suggesting the presence of 2-thiouridine derivatives. The two major lysine tRNA species (tRNA-Lys-2 and tRNA-Lys-5) were purified and their nucleoside content determined both directly and by the tritium derivative technique. Both tRNAs contain 1-methyladenosine, N-2-dimethylguanosine, 7-methylguanosine, 5-methylcytidine, pseudouridine and dihydrouridine, and tRNA-Lys-5 contains 1-methylguanosine. Neither species contain ribothymidine, although both may contain 2'-O-methyl ribothymidine. A nucleoside with ultraviolet spectral properties similar to N-4-acetylcytidine was found in tRNA-Lys-5 and a nucleoside with chromatographic properties the same as N-[9-beta-D-ribofuranosyl)-purin-6-yl-carbamoyl] threonine was found in tRNA-Lys-2. A 2-thiouridine derivative was not found in tRNA-Lys-5 using these chromatographic techniques.
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