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  • Title: The separation of 9 S RNA from avian immature red blood cells into f2c-histone mRNA and globin mRNA.
    Author: Knöchel W, Heyer I.
    Journal: Biochim Biophys Acta; 1975 Jul 23; 395(4):501-8. PubMed ID: 1148248.
    Abstract:
    9 S RNA from avian immature red blood cells was isolated from polysome-released ribonucleoprotein particles by sucrose-gradient techniques. Translation of this RNA in an Ehrlich ascites cell-free system and product analysis revealed that globin mRNA was contaminated by f2c-histone mRNA. When 9 S RNA was applied to oligo(dT)-cellulose columns a partial separation could be achieved. Poly (A)-containing globin mRNA did not contain f2c-histon mRNA, whereas the RNA which was not absorbed to oligo(dT)-cellulose contained all the f2c-histone mRNA besides substantial amounts of globin mRNA.
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