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  • Title: The composition of the insoluble collagenous matrix of bovine predentine.
    Author: Carmichael DJ, Chovelon A, Pearson CH.
    Journal: Calcif Tissue Res; 1975 Jun 18; 17(4):263-71. PubMed ID: 1148888.
    Abstract:
    Predentine obtained from bovine teeth by microdissection was extracted with EDTA and tris-NaCl solutions. The insoluble residue consisted mainly of collagen and resembled dentine collagen in overall amino acid composition. The residue differed in containing no detectable phosphoprotein, a much larger amount of collagen hexose and more non-collagenous glycoprotein, the neutral sugar composition of which was determined. Differences were also observed in the contents of reducible collagen cross-links. Half of the total phosphorus found in the predentine could not be accounted for solely by hydroxyapatite. The remainder was partly soluble and dialysable.
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