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  • Title: Isolation, nucleotide sequence and expression of a cDNA encoding feline granulocyte colony-stimulating factor.
    Author: Dunham SP, Onions DE.
    Journal: Cytokine; 2001 Jun 21; 14(6):347-51. PubMed ID: 11497496.
    Abstract:
    A cDNA encoding feline granulocyte colony stimulating factor (fG-CSF) was cloned from alveolar macrophages using the reverse transcriptase-polymerase chain reaction. The cDNA is 949 bp in length and encodes a predicted mature protein of 174 amino acids. Recombinant fG-CSF was expressed as a glutathione S-transferase fusion and purified by affinity chromatography. Biological activity of the recombinant protein was demonstrated using the murine myeloblastic cell line GNFS-60, which showed an ED50 for fG-CSF of approximately 2 ng/ml.
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