These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Role of c-fos gene in the pulmonary surfactant synthesis of cultured alveolar type II cells induced by endothelin-1].
    Author: Luo ZQ, Sun XH, Qin XQ.
    Journal: Sheng Li Xue Bao; 1999 Jun; 51(3):241-5. PubMed ID: 11498983.
    Abstract:
    The effects of endothelin-1 (ET-1) on pulmonary surfactant (PS) synthesis of cultured alveolar type II cells (AT II) were observed. The role of c-fos gene in cellular signal transduction of ET-1 was studied by antisense technology. The results showed that: (1) ET-1 enhanced [3H] choline incorporation into AT II cells in a dose-dependent manner. (2) Protein kinase (PKC) activator PMA increased [3H] choline incorporation into AT II cells, while PKC inhibitor H7 inhibited the stimulating effect of ET-1. (3) Both ET-1 and PMA could increase the level of c-Fos protein, and H7 and c-fos antisense oligonucleotides (AS ODN) could inhibit the effects induced by ET-1 on Fos protein expression and [3H] choline incorporation. (4) The release of lactic dehydrogenase (LDH) was not different among control, ET-1, antisense oligonucleotides and sense oligonucleotides groups. The above results demonstrated that ET-1 can enhance PS synthesis of AT II cells and ET-1 stimulating the expression of c-fos gene mediated by PKC is a major signal transduction pathway of modulating PS synthesis.
    [Abstract] [Full Text] [Related] [New Search]