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  • Title: Inactivating properties of recombinant ROMK2 channels expressed in mammalian cells.
    Author: Riochet DF, Mohammad-Panah R, Hebert SC, MacGregor GG, Baró I, Guihard G, Escande D.
    Journal: Biochem Biophys Res Commun; 2001 Aug 17; 286(2):376-80. PubMed ID: 11500048.
    Abstract:
    Biophysical properties of ROMK2 channel were investigated at physiological temperature, after reexpression of the recombinant ROMK2 protein in a mammalian cell expression system (COS-7). We observed that ROMK2 induced an inwardly rectifying K(+) current whether polyvalent cations were present or not. Above +10 mV, ROMK2-induced current exhibited a voltage- and time-dependent decay, consistent with an inactivation process. Inactivation of ROMK2-induced current was also seen in inside out patch from ROMK2-expressing Xenopus oocyte. In COS-7 cells, inactivation was found to account for most of the inward rectification. Mg(2+) and spermine modulated rectification by accelerating inactivation kinetics independently of membrane potential. These results establish for the first time ROMK2 properties in a mammalian cell expression system.
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