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  • Title: A quantitative method for determination of aflatoxin B in roasted corn.
    Author: Shannon GM, Shotwell OL.
    Journal: J Assoc Off Anal Chem; 1975 Jul; 58(4):743-5. PubMed ID: 1150613.
    Abstract:
    Roasting aflatoxin-contaminated corn will reduce toxin levels. A quantitative analysis for aflatoxin in roasted corn has been developed by modifying a cleanup technique for green coffee extracts approved as official first action by the AOAC. A chloroform extract is partially purified on a Florisil column, and thin layer chromatographic (TLC) plates are developed with methylene chloride-chloroform-isoamyl alcohol-formic acid (81+15+3+1). Recoveries average 101% and the sensitivity limit is 5 ppb aflatoxin B1. A 2-dimensional TLC procedure can also be used to separate the aflatoxins from background interferences.
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