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  • Title: Targeted use of exoglycosidase digestion for the structural elucidation of neutral O-linked oligosaccharides.
    Author: Xie Y, Tseng K, Lebrilla CB, Hedrick JL.
    Journal: J Am Soc Mass Spectrom; 2001 Aug; 12(8):877-84. PubMed ID: 11506219.
    Abstract:
    Exoglycosidase digestion in combination with the catalog-library approach (CLA) is used with matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) to obtain the complete structure of oligosaccharides. The CLA is a collision-induced dissociation (CID)-based method used to determine the structure of O-linked neutral oligosaccharides. It provides both linkage and stereochemical information. Exoglycosidases are used to confirm independently the validity of the CLA. In some cases, the CLA provides structural information on all but a single residue. Exoglycosidase is used to refine these structures. In this way, exoglycosidase use is targeted employing only a small number of enzymes. Exoglycosidase arrays, which have been used with N-linked oligosaccharides, is avoided despite the larger variations in structures of O-linked species.
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