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  • Title: Quantitation of tryptophan metabolites in rat feces by thin-layer chromatography.
    Author: Anderson GM.
    Journal: J Chromatogr; 1975 Feb 26; 105(2):323-8. PubMed ID: 1150778.
    Abstract:
    Indole, skatole, indole-3-acetic acid, indole-3-propionic acid, tryptamine, and free tryptophan have been extracted from rat fecal samples and quantitated by thin-layer chromatography. Additional tryptophan metabolites have been recovered in yields of 87-97% from supplemented fecal samples. Detection limits for all the compounds studied were in the 0.1 mug/g feces range. Quantitation was possible in the mug/g range with an estimated accuracy of plus or minus 10%.
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