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  • Title: Evaluation and utilization of a kinetic enzyme direct measuring photometer.
    Author: Wolf PL.
    Journal: J Clin Pathol; 1975 Jul; 28(7):587-91. PubMed ID: 1150899.
    Abstract:
    We have recently utilized a prototype model of the Beckman Enzyme Activity Analyzer System-TR in our laboratory measuring various serum enzyme activities which include: alkaline phosphatase (ALP), E.C.3.1.3.1; creatine kinase (CK), E.C.2.7.3.2; hydroxybutyrate dehydrogenase (HBD), E.C.1.1.1.30; lactate dehydrogenase (LD), E.C.1.1.1.27; aspartate transaminase (AST), E.C.2.6.1.1; and alanine transaminase (ALT), E.C.2.6.1.2. Precision was found to be good. Sample activities could be measured as high as 1000 IU/1. The carryover studies fell within 2 SD of the means of the enzyme control studies. Coefficients of variation for ALP and CK were in the ranges of 0-40-2-14% and 0-52-4-30%, respectively. Correlation studies were done with GemSAEC and Gilford 300 N Spectrophotometer and the results were accurate, precise, and reproducible.
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