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  • Title: High-pressure liquid chromatographic analysis of penicillin G potassium and its degradation products.
    Author: Blaha JM, Knevel AM, Hem SL.
    Journal: J Pharm Sci; 1975 Aug; 64(8):1384-6. PubMed ID: 1151712.
    Abstract:
    An anion-exchange high-pressure liquid chromatographic system capable of separating penicillin G potassium from five of its degradation products was developed. The retention times were: penicillin G potassium, 17.5 min; DL-penicillamine, 4.5 min; benzylpenilloic acid, 7.0 and 8.0 min; benzylpenamaldic acid, 13.0 min; benzylpenicilloic acid, 19.5 min; and benzylpenillic acid, 22.0 min. In addition, the system permits quantification using linear calibration curves.
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